신제품소개
연구 및 진단 분야
의료 산업용 소재 분야
화장품 분야
진료재료 및 소모품 분야
기타분야
Biolipidure Series
Contact Lens Raw Materials
Lipidure CM Series
Medical Nylon
Medical Silicone
Medical TPU
Medical Tube Extrusion
Silicone for Aerospace
Water soluble Urethane
연구 및 진단 분야
| Features | No danger of biohazards |
|---|---|
| No difference in lots | |
| Suppression of non-specific adsorption | |
| Stabilization of immobilized antibody | |
| Stabilization of enzyme-antibody conjugate | |
| Enhancement of aggregation reaction | |
| Enhancement of enzyme-substrate reaction | |
| Enhancement of accuracy | |
| Reduction of CV value ([standard deviation] / [average] x 100) | |
| Improvement of particle dispersiveness |
| General Usage |
Can be supplied as 5 wt% aqueous solution
Do not use Biolipidure™ directly, because it is not a buffer solution. |
|---|---|
| Can be used in ELISA, Western Blotting, Immuno-histochemistry. | |
|
※ Dilute with a buffer solution.
It is recommended that the conc. of Biolipidure is used as 1.0 wt% - 0.01 wt%. |
| Solid phase : 96-well plate (Maxisorp F96, Nunc) |
| Immobilized antibody : anti (mouse IgG)IgG |
| Antigen : non |
| Enzyme-IgG conjugate : HRP-Anti (mouse IgG) IgG conjugate |
| Substrate : o-phenylenediamine dihydrochloride Detection: 492 nm |
| Experiment condition : | |
| - | Immobilization of anti (mouse IgG)IgG |
| - | Blocking by 0.1% (1 mg/mL) BIOLIPIDURE and 1 mg/mL BSA |
| - | Dispensation of HRP-Anti (mouse IgG) IgG conjugate |
| - | Dispensation of o-phenylenediamine dihydrochloride |
| - | Measurement of absorbance at 492 nm |
| Solid phase : 96-well plate (Maxisorp F96, Nunc) |
| Immobilized antibody : anti (mouse IgG)IgG |
| Antigen : mouse IgG |
| Enzyme-IgG conjugate : HRP-Anti (mouse IgG) IgG conjugate |
| Substrate : o-phenylenediamine dihydrochloride |
| Detection : 492 nm |
| Experiment condition : | |
| - | Immobilization of anti (mouse IgG)IgG |
| - | Blocking by by 0.1% BIOLIPIDURE and 0.1 % BSA |
| - | Storage at 40 °Ｃ : 0, 4, 7, 14 and 20 day |
| - | Dispensation of mouse IgG |
| - | Dispensation of HRP-Anti (mouse IgG) IgG conjugate |
| - | Dispensation of o-phenylenediamine dihydrochloride |
| - | Measurement of absorbance at 492 nm |
| Solid phase : 96-well plate (Maxisorp F96, Nunc) |
| Immobilized antibody : anti (mouse IgG)IgG |
| Antigen : mouse IgG |
| Enzyme-IgG conjugate : HRP-Anti (mouse IgG) IgG conjugate |
| Substrate : o-phenylenediamine dihydrochloride |
| Detection : 492 nm |
| Experiment condition : | |
| - | Preparation of plate (immobilized antibody and reacted antigen) |
| - | Dispensation of 1 % BIOLIPIDURE and BSA into HRP-IgG conjugate |
| - | Storage at 25 °Ｃ: 0, 3, 7, 14, 24 and 37 day |
| - | Dispensation of HRP-Anti (mouse IgG) IgG |
| - | Dispensation of o-phenylenediamine dihydrochloride |
| - | Measurement of absorbance at 492 nm |
| Analysis : 0.8 mg/dL CRP using 7070 Auto Analyzer (HITACH) |
| Measurement: latex turbidly immunoassay |
| Experiment condition : | |
| - | Dispensation of 1% BIOLIPIDURE, PEG and PVP into R1 reagent |
| PEG : polyethylene glycol (Mw = 6,000) |
| PVP : polyvinylpyrrolidone (Mw = 40,000) |
| - Biolipidure™ features - | |
| - | No danger of biohazards and no difference in lots |
| - | Improvement of stability, accuracy, reproducibility and sensitivity. |
| - | Can be supplied as 5 wt% aqueous solution |
| - Usage of Biolipidure™ as a mobile phase - | |
| #1 | Prepare the buffer solution including one wt% of Biolipidure. |
| #2 | Dissolve the sample (For example; mucosa) in the above buffer. |
| #3 | Put the diluted sample on the solid phase of immunochromatography. |
| - Usage of Biolipidure™ as a solid phase - | |
| #1 | Prepare the solution of antibodies-conjugated gold colloids. |
| #2 | Draw the line using the colloids solution on the solid phase of immunochromatography. |
| #3 | Soak the solid phase in the buffer solution including one wt% of Biolipidure. |
| #4 | Pick up the solid phase and dry it. You can get the solid phase blocked by Biolipidure. |
