Features | Hemo-compatibility |
---|---|
Anti-adhesion of proteins and cells | |
Anti-denaturing of proteins | |
Anti-activation of cells | |
Usage | Dissolve Lipidure¢ç-CM in ethanol and prepare a 0.5wt% ethanol solution. |
Dip the base material into the coating solution for 1 min. | |
Dry the coated material at room temperature or one hour at 50ーC. | |
Substrates | Lipidure¢ç-CM can be coated on the following substrates : |
Plastics (PET, PMMA, PSt, PU, PC, PE, PP) | |
Glass, Ceramics and Metals (SUS, titanium, gold) |
Lipidure-CM5206 |
m=3, n=7, Alkyl= n-Butyl |
Lipidure-CM5206 is equivalent to ¡°PMB30¡± |
in the Professors Nobuo Nakabayashi¡¯s and Kazuhiko Ishihara¡¯s literatures. |
References | |
K. | Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 28, 1347-1355(1994) |
K. | Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 39, 323-330(1998) |
K. | Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 64, 411-416(2003) |
K. | Nakabayashi, Macromol. Symp. 245-246, 591-598(2006) |
Experimental procedure | |
- | Dispensation of HRP-IgG conjugate solution into each well |
- | Incubation for 1 h at 25¡ÆC |
- | Washing three times with Dulbeco¡¯ PBS including 0.05% of Tween20. |
- | Dispensation of 3,3¡¯,5,5¡¯ - tetramethylbenzidine solution |
- | Measurement of absorbance at 450 nm |
The Lipidure¢ç treatment was able to reduce the amount of IgG absorption to below 10% of non-treated pleat. The same results were observed with other proteins |
Experimental procedure | |
- | Seeding of macrophage into each well (3.3 x 105 cells/well) |
- | Washing with Dulbeco¡¯ PBS |
- | Fixing with glutaraldehyde solution |
- | Observation by SEM |
The activation and adhesion of macrophage was prevented by Lipidure¢ç treatment. Also, the Lipidure¢ç treatment was effective for other cells. |
£Coating£ |
PC surface can be made by coating with Lipidure¢ç-CM. The following figure shows the relationship between ¡°he number of times the coating was applied¡±and ¡°he thickness of the coating film¡± The thickness of the Lipidure¢ç-CM coating film is calculated by weight measurement. These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure¢ç in the coating solution. |
£Safety Data£ |
We have the following toxicological data for Lipidure¢ç-CM5206. Acute oral toxicity, Skin irritation, Skin sensitization, Mutagenic potential (Ames Test), Hemolysis test, Endotoxin test |
Reactive Lipidure
Product | Reactive Group X | Packing Size | Appearence |
Lipidure NH01 | NH2 | 10, 100g | 5wt% aqueous solution |
Lipidure CR2001 | UV Curing | 1, 10, 10g | White powder |
Lipidure CR3001 | Alkoxy silane | 1, 10, 10g | White powder |