BiolipidureTM
- Features -
-No danger of biohazards
-No difference in lots
-Suppression of non-specific adsorption
-Stabilization of immobilized antibody
-Stabilization of enzyme-antibody conjugate
-Enhancement of aggregation reaction
-Enhancement of enzyme-substrate reaction
-Enhancement of accuracy
-Reduction of CV value ([standard deviation] / [average] x 100)
-Improvement of particle dispersiveness
- General Usage -
Can be supplied as 5 wt% aqueous solution
Do not use Biolipidure TM directly, because it is not a buffer solution.
Can be used in ELISA, Western Blotting, Immuno-histochemistry.
※ Dilute with a buffer solution.
It is recommended that the conc. of Biolipidure is used as 1.0 wt% - 0.01 wt%.
※ Store at 4 ﾟC.
Note 1) Hydrophilicity :103 > 405, 502, 702 > 203, 802, 1201, 1301 > 206, 1002
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Solid phase: 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody: anti (mouse IgG)IgG
Antigen: non
Enzyme-IgG conjugate: HRP-Anti (mouse IgG) IgG conjugate
Substrate: o-phenylenediamine dihydrochloride
Detection: 492 nm
Experiment condition:
-Immobilization of anti (mouse IgG)IgG
-Blocking by 0.1% (1 mg/mL) BIOLIPIDURE and 1 mg/mL BSA
-Dispensation of HRP-Anti (mouse IgG) IgG conjugate
-Dispensation of o-phenylenediamine dihydrochloride
-Measurement of absorbance at 492 nm
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Solid phase: 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody: anti (mouse IgG)IgG
Antigen: mouse IgG
Enzyme-IgG conjugate: HRP-Anti (mouse IgG) IgG conjugate
Substrate: o-phenylenediamine dihydrochloride
Detection: 492 nm
Experiment condition:
-Immobilization of anti (mouse IgG)IgG
-Blocking by by 0.1% BIOLIPIDURE and 0.1 % BSA
-Storage at 40 °Ｃ : 0, 4, 7, 14 and 20 day
-Dispensation of mouse IgG
-Dispensation of HRP-Anti (mouse IgG) IgG conjugate
-Dispensation of o-phenylenediamine dihydrochloride
-Measurement of absorbance at 492 nm
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Solid phase: 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody: anti (mouse IgG)IgG
Antigen: mouse IgG
Enzyme-IgG conjugate: HRP-Anti (mouse IgG) IgG conjugate
Substrate: o-phenylenediamine dihydrochloride
Detection: 492 nm
Experiment condition:
-Preparation of plate (immobilized antibody and reacted antigen)
-Dispensation of 1 % BIOLIPIDURE and BSA into HRP-IgG conjugate
-Storage at 25 °Ｃ: 0, 3, 7, 14, 24 and 37 day
-Dispensation of HRP-Anti (mouse IgG) IgG
-Dispensation of o-phenylenediamine dihydrochloride
-Measurement of absorbance at 492 nm
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Analysis: 0.8 mg/dL CRP using 7070 Auto Analyzer (HITACH)
Measurement: latex turbidly immunoassay
Experiment condition:
-Dispensation of 1% BIOLIPIDURE, PEG and PVP into R1 reagent
PEG: polyethylene glycol (Mw = 6,000)
PVP: polyvinylpyrrolidone (Mw = 40,000)
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BiolipidureTM for Immunochromatography
- BiolipidureTM features -
-No danger of biohazards and no difference in lots
-Improvement of stability, accuracy, reproducibility and sensitivity.
-Can be supplied as 5 wt% aqueous solution
- Usage of BiolipidureTM as a mobile phase -
#1 Prepare the buffer solution including one wt% of Biolipidure.
#2 Dissolve the sample (For example; mucosa) in the above buffer.
#3 Put the diluted sample on the solid phase of immunochromatography.
- Usage of BiolipidureTM as a solid phase -
#1 Prepare the solution of antibodies-conjugated gold colloids.
#2 Draw the line using the colloids solution on the solid phase of immunochromatography.
#3 Soak the solid phase in the buffer solution including one wt% of Biolipidure.
#4 Pick up the solid phase and dry it. You can get the solid phase blocked by Biolipidure.
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