면역 반응을 위한 blocking 전용 시약으로 매우 우수한 효과를 발휘하는 제품입니다.
NB4025 제품은 특히 사람 혈청 IgG에 효과가 우수합니다.
NB 시리즈는 250ml working solution 형태로 판매되고 있으며 희석과정 없이 바로 사용 가능합니다.
일반적으로 이 제품들은 블록킹한 후 건조처리할 것을 추천합니다.
(사용 조건에 따라 예외가 있기 때문에 사용 용도 등을 저희 담당자와 상담해 주시기 바랍니다.)
Blocking reagents NB2025, NB3025
- Features -
-Blocking reagent for immunoassay
-No bio-derived materials
-Excellent effect of non-specific binding
－Usage－
-Use it like a usual blocking reagent
-Use from 1- to 25-fold dilution ---we recommend 5-fold dilution by saline---
-Recommended for use in dry-treatment after blocking
－Precautions for use－
The product contain 0.09% sodium azide as a preservative. Be careful not to ingest it by mistake and take care when disposing of it.
－Comparison of results－
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Immunoplate well (Maxisorp F96, Nunc) was treated with a 5-fold diluted blocking reagent (at room temp., 15-60 min.), POD-conjugate solution was added and incubated at 37°C for 120 min. After washing with PBS containing 0.05% tween20 (PBST), we added a color reagent for POD (H2O2-TMBZ system) and evaluated the amount of POD which remained in the well. There was no significant difference between Casein and NB2025, NB3025. |
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Immunoplate well (Maxisorp F96, Nunc) was immobilized with anti-mouse-IgG-antibody. After blocking with 5-fold diluted blocking reagent (at room temp., 120 min.), the plate which was de-air-packed in an aluminum bag, was stored at 50. The activity of the immobilized-antibody was evaluated with a sandwich-ELISA system. |
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After blocking (see above), the POD-conjugate diluted with PBST was added and incubated at 37°C for 120 min. After washing with PBST, we added a color reagent for POD (H2O2-TMBZ system) and evaluated the amount of POD that remained in the well. |
Blocking reagents NB4025
- Features -
-Blocking reagent for immunoassay
-No bio-derived materials
-Excellent effect of non-specific binding, especially human IgG in sera.
－Usage－
-Use it like a usual blocking reagent
-Use from 1- to 25-fold dilution ---we recommend 5-fold dilution by saline---
-Recommended for use in dry-treatment after blocking
－Precautions for use－
The product contain 0.09% sodium azide as a preservative. Be careful not to ingest it by mistake and take care when disposing of it.
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Methods
After blocking an immuno-plate(5-fold dilution by saline,at 37°C for 2hrs),a human sera sample(20-fold dilution by PBS)was added. After incubation for 2hrs at 37°C,the plate was washed with 0.05% tween20-PBS.Then POD-labeled antibodies were added and it was reacted at 37°C for 2hrs. After washing,a coloring reaction(TMBZ) was conducted.The non-specific binding substances of the sera were analyzed.
Non specific binding of human serum IgG was suppressed efficiently.
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