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PRODUCT
신제품소개
연구 및 진단 분야
의료 산업용 소재 분야
화장품 분야
진료재료 및 소모품 분야
기타분야
Biolipidure Series
Contact Lens Raw Materials
Lipidure CM Series
Medical Nylon
Medical Silicone
Medical TPU
Medical Tube Extrusion
Water soluble Urethane
연구 및 진단 분야
Biolipidure™
Features No danger of biohazards
No difference in lots
Suppression of non-specific adsorption
Stabilization of immobilized antibody
Stabilization of enzyme-antibody conjugate
Enhancement of aggregation reaction
Enhancement of enzyme-substrate reaction
Enhancement of accuracy
Reduction of CV value ([standard deviation] / [average] x 100)
Improvement of particle dispersiveness
General Usage Can be supplied as 5 wt% aqueous solution
Do not use Biolipidure™ directly, because it is not a buffer solution.
Can be used in ELISA, Western Blotting, Immuno-histochemistry.
※ Dilute with a buffer solution.
It is recommended that the conc. of Biolipidure is used as 1.0 wt% - 0.01 wt%.
※ Store at 4 ゚C.
Note 1) Hydrophilicity :103 > 405, 502, 702 > 203, 802, 1201, 1301 > 206, 1002
Solid phase : 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody : anti (mouse IgG)IgG
Antigen : non
Enzyme-IgG conjugate : HRP-Anti (mouse IgG) IgG conjugate
Substrate : o-phenylenediamine dihydrochloride Detection: 492 nm
Experiment condition :
- Immobilization of anti (mouse IgG)IgG
- Blocking by 0.1% (1 mg/mL) BIOLIPIDURE and 1 mg/mL BSA
- Dispensation of HRP-Anti (mouse IgG) IgG conjugate
- Dispensation of o-phenylenediamine dihydrochloride
- Measurement of absorbance at 492 nm
Solid phase : 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody : anti (mouse IgG)IgG
Antigen : mouse IgG
Enzyme-IgG conjugate : HRP-Anti (mouse IgG) IgG conjugate
Substrate : o-phenylenediamine dihydrochloride
Detection : 492 nm
Experiment condition :
- Immobilization of anti (mouse IgG)IgG
- Blocking by by 0.1% BIOLIPIDURE and 0.1 % BSA
- Storage at 40 °C : 0, 4, 7, 14 and 20 day
- Dispensation of mouse IgG
- Dispensation of HRP-Anti (mouse IgG) IgG conjugate
- Dispensation of o-phenylenediamine dihydrochloride
- Measurement of absorbance at 492 nm
Solid phase : 96-well plate (Maxisorp F96, Nunc)
Immobilized antibody : anti (mouse IgG)IgG
Antigen : mouse IgG
Enzyme-IgG conjugate : HRP-Anti (mouse IgG) IgG conjugate
Substrate : o-phenylenediamine dihydrochloride
Detection : 492 nm
Experiment condition :
- Preparation of plate (immobilized antibody and reacted antigen)
- Dispensation of 1 % BIOLIPIDURE and BSA into HRP-IgG conjugate
- Storage at 25 °C: 0, 3, 7, 14, 24 and 37 day
- Dispensation of HRP-Anti (mouse IgG) IgG
- Dispensation of o-phenylenediamine dihydrochloride
- Measurement of absorbance at 492 nm
Analysis : 0.8 mg/dL CRP using 7070 Auto Analyzer (HITACH)
Measurement: latex turbidly immunoassay
Experiment condition :
- Dispensation of 1% BIOLIPIDURE, PEG and PVP into R1 reagent
PEG : polyethylene glycol (Mw = 6,000)
PVP : polyvinylpyrrolidone (Mw = 40,000)
Biolipidure™ for Immunochromatography
- Biolipidure™ features -
- No danger of biohazards and no difference in lots
- Improvement of stability, accuracy, reproducibility and sensitivity.
- Can be supplied as 5 wt% aqueous solution
- Usage of Biolipidure™ as a mobile phase -
#1 Prepare the buffer solution including one wt% of Biolipidure.
#2 Dissolve the sample (For example; mucosa) in the above buffer.
#3 Put the diluted sample on the solid phase of immunochromatography.
- Usage of Biolipidure™ as a solid phase -
#1 Prepare the solution of antibodies-conjugated gold colloids.
#2 Draw the line using the colloids solution on the solid phase of immunochromatography.
#3 Soak the solid phase in the buffer solution including one wt% of Biolipidure.
#4 Pick up the solid phase and dry it. You can get the solid phase blocked by Biolipidure.
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