신제품소개
연구 및 진단 분야
의료 산업용 소재 분야
화장품 분야
진료재료 및 소모품 분야
기타분야
Biolipidure Series
Contact Lens Raw Materials
Lipidure CM Series
Medical Nylon
Medical Silicone
Medical TPU
Medical Tube Extrusion
Silicone for Aerospace
Water soluble Urethane
연구 및 진단 분야
| Features | Hemo-compatibility |
|---|---|
| Anti-adhesion of proteins and cells | |
| Anti-denaturing of proteins | |
| Anti-activation of cells | |
| Usage | Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution. |
| Dip the base material into the coating solution for 1 min. | |
| Dry the coated material at room temperature or one hour at 50ｰC. | |
| Substrates | Lipidure®-CM can be coated on the following substrates : |
| Plastics (PET, PMMA, PSt, PU, PC, PE, PP) | |
| Glass, Ceramics and Metals (SUS, titanium, gold) |
| Lipidure-CM5206 |
| m=3, n=7, Alkyl= n-Butyl |
| Lipidure-CM5206 is equivalent to “PMB30” |
| in the Professors Nobuo Nakabayashi’s and Kazuhiko Ishihara’s literatures. |
| References | |
| K. | Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 28, 1347-1355(1994) |
| K. | Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 39, 323-330(1998) |
| K. | Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 64, 411-416(2003) |
| K. | Nakabayashi, Macromol. Symp. 245-246, 591-598(2006) |
| Experimental procedure | |
| - | Dispensation of HRP-IgG conjugate solution into each well |
| - | Incubation for 1 h at 25°C |
| - | Washing three times with Dulbeco’ PBS including 0.05% of Tween20. |
| - | Dispensation of 3,3’,5,5’ - tetramethylbenzidine solution |
| - | Measurement of absorbance at 450 nm |
| The Lipidure® treatment was able to reduce the amount of IgG absorption to below 10% of non-treated pleat. The same results were observed with other proteins |
| Experimental procedure | |
| - | Seeding of macrophage into each well (3.3 x 105 cells/well) |
| - | Washing with Dulbeco’ PBS |
| - | Fixing with glutaraldehyde solution |
| - | Observation by SEM |
| The activation and adhesion of macrophage was prevented by Lipidure® treatment. Also, the Lipidure® treatment was effective for other cells. |
| －Coating－ |
| PC surface can be made by coating with Lipidure®-CM. The following figure shows the relationship between “he number of times the coating was applied”and “he thickness of the coating film” The thickness of the Lipidure®-CM coating film is calculated by weight measurement. These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure® in the coating solution. |
| －Safety Data－ |
| We have the following toxicological data for Lipidure®-CM5206. Acute oral toxicity, Skin irritation, Skin sensitization, Mutagenic potential (Ames Test), Hemolysis test, Endotoxin test |
