Chemical structure

Coating Method

Available substrate

Lipidure-CM5206 can be coated on the following materials

Feature

・Suppression of protein adsorption

<<Method>>
1. Prepare well plate coated with Lipidure®-CM5206
2. Add HRP-IgG conjugate solution into each well
3. Incubate for 1 hr at 25 ºC
4. Rinse three times with 0.05 % Tween20/Dulbeco’s PBS
5. Add of 3,3’,5,5’-tetramethylbenzidine solution into each well
6. Measure absorbance at 450 nm

・Suppression of cell adsorption

<<Method>>
1. Prepare well plate coated with Lipidure®-CM5206
2. Seed macrophage in well plate at 3.3x10⁵ cells/well
3. Incubate for 1 hr at 37 ºC in 5% CO₂ atmosphere
4. Rinse off with Dulbeco’s PBS
5. Fix with glutaraldehyde and Observation by SEM

・Hemo-compatibility

<<Method>>
1. Prepare artificial vascular coated with Lipidure®-CM5206
2. Rabbit blood was flowed in Lipidure®-CM5206 treated artificial vascular
3. Rinse artificial vascular with buffer solution
4. Take photo of inside of artificial vascular